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Media type:
E-Article
Title:
Post-transcriptional regulation of microRNA expression
Contributor:
Obernosterer, Gregor;
Leuschner, Philipp J.F.;
Alenius, Mattias;
Martinez, Javier
Published:
Cold Spring Harbor Laboratory, 2006
Published in:
RNA, 12 (2006) 7, Seite 1161-1167
Language:
English
DOI:
10.1261/rna.2322506
ISSN:
1355-8382;
1469-9001
Origination:
Footnote:
Description:
microRNAs (miRNAs) are endogenous, noncoding ∼22-nucleotide RNA molecules that have recently emerged as fundamental, post-transcriptional regulators of cognate target gene expression. Many mammalian miRNAs are expressed in a tissue-specific manner, a phenomenon that has so far been attributed to transcriptional regulation. We here show by Northern blots and in situ hybridization experiments that the expression of mammalian miRNAs can be regulated at the post-transcriptional level. In particular, miR-138 is spatially restricted to distinct cell types, while its precursor, pre-miR-138-2, is ubiquitously expressed throughout all tissues analyzed. Furthermore, pre-miR-138-2 is exported from the nucleus to the cytoplasm, suggesting that cleavage of this pre-miRNA by Dicer is restricted to certain tissues and cell types. Thus, differential processing of pre-miRNAs might be an alternative mechanism to control miRNA function.