• Media type: E-Article
  • Title: Design and development of volume phase holographic grating based digital holographic interferometer for label-free quantitative cell imaging
  • Contributor: Rastogi, Vivek; Agarwal, Shilpi; Dubey, Satish Kumar; Khan, Gufran Sayeed; Shakher, Chandra
  • imprint: Optica Publishing Group, 2020
  • Published in: Applied Optics, 59 (2020) 12, Seite 3773
  • Language: English
  • DOI: 10.1364/ao.387620
  • ISSN: 1559-128X; 2155-3165
  • Keywords: Atomic and Molecular Physics, and Optics ; Engineering (miscellaneous) ; Electrical and Electronic Engineering
  • Origination:
  • Footnote:
  • Description: <jats:p>In this paper, a volume phase holographic optical element based digital holographic interferometer is designed and used for quantitative phase imaging of biological cells [white blood cells, red blood cells, platelets, and <jats:italic toggle="yes">Staphylococcus aureus</jats:italic> (<jats:italic toggle="yes">S. aureus</jats:italic>) bacteria cells]. The experimental results reveal that sharp images of the <jats:italic toggle="yes">S. aureus</jats:italic> bacteria cells of the order of <jats:inline-formula> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"> <mml:mrow class="MJX-TeXAtom-ORD"> <mml:mo>∼<!-- ∼ --></mml:mo> </mml:mrow> <mml:mrow class="MJX-TeXAtom-ORD"> <mml:mn>1</mml:mn> </mml:mrow> <mml:mspace width="thickmathspace" /> <mml:mrow class="MJX-TeXAtom-ORD"> <mml:mtext>µ<!-- µ --></mml:mtext> <mml:mrow class="MJX-TeXAtom-ORD"> <mml:mi mathvariant="normal">m</mml:mi> </mml:mrow> </mml:mrow> </mml:math> </jats:inline-formula> can be clearly seen. The volume phase holographic grating will remove the stray light from the system reaching toward the grating and will minimize the coherent noise (speckle noise). This will improve the sharpness in the image reconstructed from the recorded digital hologram.</jats:p>