Denton, Karin J.;
Bergeron, Christine;
Klement, Petra;
Trunk, Marcus J.;
Keller, Thomas;
Ridder, Ruediger
The Sensitivity and Specificity of p16INK4a Cytology vs HPV Testing for Detecting High-Grade Cervical Disease in the Triage of ASC-US and LSIL Pap Cytology Results
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Media type:
E-Article
Title:
The Sensitivity and Specificity of p16INK4a Cytology vs HPV Testing for Detecting High-Grade Cervical Disease in the Triage of ASC-US and LSIL Pap Cytology Results
Contributor:
Denton, Karin J.;
Bergeron, Christine;
Klement, Petra;
Trunk, Marcus J.;
Keller, Thomas;
Ridder, Ruediger
Published:
Oxford University Press (OUP), 2010
Published in:
American Journal of Clinical Pathology, 134 (2010) 1, Seite 12-21
Language:
English
DOI:
10.1309/ajcp3cd9ykyfjdql
ISSN:
1943-7722;
0002-9173
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title><jats:p>We analyzed the performance of p16INK4a immunocytochemistry on a series of 810 retrospectively collected atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL) cases with available biopsy follow-up data, including 94 cases of cervical intraepithelial neoplasia (CIN) 2 and 128 cases of CIN 3. Human papillomavirus (HPV) testing was performed from the same residual liquid-based cytologic specimen, and results for both tests were correlated with histologic follow-up data. Sensitivity values for high-grade CIN (HGCIN) confirmed on biopsy within 6 months were 92.6% (ASC-US) and 92.2% (LSIL) for cytotechnologists’ reviews of p16 cytology and 90.1% (ASC-US) and 95.7% (LSIL) for HPV testing. Sensitivity rates of initial pathologists’ reviews were slightly lower, 76.4% to 80.1%, with levels comparable to cytotechnologists’ results after adjudication. The specificity of p16 cytology for HGCIN detection was significantly higher than for HPV testing for cytotechnologists and pathologists: 63.2% to 71.1% (p16 cytology) vs 37.8% for HPV in ASC-US (P &lt; .001) and 37.3% to 53.3% (p16 cytology) vs 18.5% for HPV in LSIL (P &lt; .001). This evaluation of the diagnostic performance of p16 cytology confirms the potential of this stain for the efficient triage of ASC-US and LSIL cytologic results.</jats:p>