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Media type:
E-Article
Title:
Screening for novel peptides specifically binding to the surface of ectopic endometrium cells by phage display
Contributor:
Linzhi, Yan;
Dan, Cao;
Arhin, Samuel Kofi;
Ledan, Wang;
Wenju, Li;
Jieqiang, Lv
Published:
CMB Association, 2018
Published in:
Cellular and Molecular Biology, 64 (2018) 11, Seite 36-40
Language:
Not determined
DOI:
10.14715/cmb/2018.64.11.7
ISSN:
1165-158X;
0145-5680
Origination:
Footnote:
Description:
A 7-mer phage display library was employed to isolate novel peptides that specifically bind to ectopic endometrium in vitro. Phage display technology with biopanning and rapid analysis of selective interactive ligands between ectopic and eutopic endometrium cells was utilized. After 5 rounds of biopanning, 50 phage clones were randomly selected and analyzed by enzyme-linked immunosorbent assay and DNA sequencing. A peptide-competitive inhibition assay was performed to identify the affinity of positive phages toward ectopic endometrium cells. The most enriched polypeptide RTRLHTR showed higher affinity toward ectopic endometrium cells.The polypeptide RTRLHTR screened by phage display technology may offer a new direction for early diagnosis and treatment of endometriosis.