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Media type:
E-Article
Title:
Fecal microbiota transplantation decreases intestinal loads of multi-drug resistant Pseudomonas aeruginosa in murine carriers
Contributor:
Mrazek, Katharina;
Bereswill, Stefan;
Heimesaat, Markus M.
imprint:
Akademiai Kiado Zrt., 2019
Published in:European Journal of Microbiology and Immunology
Language:
Not determined
DOI:
10.1556/1886.2019.00002
ISSN:
2062-8633
Origination:
Footnote:
Description:
<jats:p>Intestinal carriage of multi-drug resistant (MDR) Gram-negative bacteria including <jats:italic>Pseudomonas aeruginosa</jats:italic> (Psae) constitutes a pivotal prerequisite for subsequent fatal endogenous infections in patients at risk. We here addressed whether fecal microbiota transplantation (FMT) could effectively combat MDR-Psae carriage. Therefore, secondary abiotic mice were challenged with MDR-Psae by gavage. One week later, mice were subjected to peroral FMT from either murine or human donors on 3 consecutive days. Irrespective of murine or human origin of fecal transplant, intestinal MDR-Psae loads decreased as early as 24 h after the initial FMT. Remarkably, the murine FMT could lower intestinal MDR-Psae burdens by approximately 4 log orders of magnitude within 1 week. In another intervention study, mice harboring a human gut microbiota were perorally challenged with MDR-Psae and subjected to murine FMT on 3 consecutive days, 1 week later. Strikingly, within 5 days, murine FMT resulted in lower loads and carrier rates of MDR-Psae in mice with a human gut microbiota. In conclusion, FMT might be a promising antibiotics-independent option to combat intestinal MDR-Psae carriage and thus prevent from future endogenous infections of patients at risk.</jats:p>