Importance of cortactin for efficient epithelial NF-ĸB activation by Helicobacter pylori, Salmonella enterica and Pseudomonas aeruginosa, but not Campylobacter spp
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Media type:
E-Article
Title:
Importance of cortactin for efficient epithelial NF-ĸB activation by Helicobacter pylori, Salmonella enterica and Pseudomonas aeruginosa, but not Campylobacter spp
Published in:European Journal of Microbiology and Immunology
Language:
Not determined
DOI:
10.1556/1886.2021.00023
ISSN:
2062-509X;
2062-8633
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title>
<jats:p>Transcription factors of the nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF-ĸB) family control important signaling pathways in the regulation of the host innate immune system. Various bacterial pathogens in the human gastrointestinal tract induce NF-ĸB activity and provoke pro-inflammatory signaling events in infected epithelial cells. NF-ĸB activation requires the phosphorylation-dependent proteolysis of inhibitor of ĸB (IĸB) molecules including the NF-ĸB precursors through ubiquitin-mediated proteolysis. The canonical NF-ĸB pathway merges on IĸB kinases (IKKs), which are required for signal transduction. Using CRISPR-Cas9 technology, secreted embryonic alkaline phosphatase (SEAP) reporter assays and cytokine enzyme-linked immunosorbent assay (ELISA), we demonstrate that the actin-binding protein cortactin is involved in NF-ĸB activation and subsequent interleukin-8 (IL-8) production upon infection by <jats:italic>Helicobacter pylori</jats:italic>, <jats:italic>Salmonella enterica</jats:italic> and <jats:italic>Pseudomonas aeruginosa</jats:italic>. Our data indicate that cortactin is needed to efficiently activate the c-Sarcoma (Src) kinase, which can positively stimulate NF-ĸB during infection. In contrast, cortactin is not involved in activation of NF-ĸB and IL-8 expression upon infection with <jats:italic>Campylobacter</jats:italic> species <jats:italic>C. jejuni</jats:italic>, <jats:italic>C. coli</jats:italic> or <jats:italic>C. consisus</jats:italic>, suggesting that <jats:italic>Campylobacter</jats:italic> species pluralis (spp.) induce a different signaling pathway upstream of cortactin to trigger the innate immune response.</jats:p>