Hydrophilic Polystyrene-Polyoxyethylene Graft Polymer Beads as Carrier of Antigenic Peptides for in vivo and in vitro Immunization Techniques: Applications to the Non-Catalytic Zinc Loop of HLADH Isozymes and to Histone Fragments
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Media type:
E-Article
Title:
Hydrophilic Polystyrene-Polyoxyethylene Graft Polymer Beads as Carrier of Antigenic Peptides for in vivo and in vitro Immunization Techniques: Applications to the Non-Catalytic Zinc Loop of HLADH Isozymes and to Histone Fragments
Description:
Polystyrene-polyethyleneglycol tentacle polymers (PS–PEG) are a novel class of graft copolymer beads based on a polystyrene matrix grafted with a brush-like layer of polyoxyethylene tentacles [9-12]. Immunogenic materials were obtained by synthesizing on tentacle polymer-bound amino derivatives as a solid support the following haptens: The C-terminal sequence 187—211 KPKAA KPKAA KPKAA KPKAA KPKKA APKKK and the N-terminal sequence 3-31 APAAP AAAPP AEKTP VKKKA AKKPA GA of histone H 1 (calf thymus), as well as the sequences 93-116 of the horse liver alcohol dehydrogenase EE (FTPQC GKCRV CKHPE GNFCL KNDL) and SS (FIPQC GKCSV CKHPE GNLCL KNSL) isozymes (Jörnvall (1970) Eur. J. Biochem. 16, 41-49), respectively. These materials proved to be efficient immunogens both in vivo and in vitro, showing excellent biocompatibility compared to other solid hapten carriers. Tentacle-based immunogens are generally available by standard synthetic procedures either by in situ synthesis of hapten molecules on or by covalent attachment of available antigens or haptens to the beads. Advantageous is the possibility to synthesize the peptide on the tentacle polymer, and to use the solid phase bound peptide directly as hapten. The beads serve as carriers, and no separate splitting off the peptide and binding to another carrier is necessary.