• Media type: E-Article
  • Title: A novel NMDA receptor test model based on hiPSC-derived neural cells
  • Contributor: Disse, Paul; Aymanns, Isabel; Ritter, Nadine; Peischard, Stefan; Korn, Lisanne; Wiendl, Heinz; Pawlowski, Matthias; Kovac, Stjepana; Meuth, Sven G.; Budde, Thomas; Strutz-Seebohm, Nathalie; Wünsch, Bernhard; Seebohm, Guiscard
  • Published: Walter de Gruyter GmbH, 2023
  • Published in: Biological Chemistry, 404 (2023) 4, Seite 267-277
  • Language: English
  • DOI: 10.1515/hsz-2022-0216
  • ISSN: 1431-6730; 1437-4315
  • Keywords: Clinical Biochemistry ; Molecular Biology ; Biochemistry
  • Origination:
  • Footnote:
  • Description: Abstract N-Methyl-D-aspartate receptors (NMDARs) are central for learning and information processing in the brain. Dysfunction of NMDARs can play a key role in the pathogenesis of neurodegeneration and drug addiction. The development of selective NMDAR modulators represents a promising strategy to target these diseases. Among such modulating compounds are ifenprodil and its 3-benzazepine derivatives. Classically, the effects of these NMDAR modulators have been tested by techniques like two-electrode voltage clamp (TEVC), patch clamp, or fluorescence-based assays. However, testing their functional effects in complex human systems requires more advanced approaches. Here, we established a human induced pluripotent stem cell-derived (hiPSC-derived) neural cell system and proved its eligibility as a test system for investigating NMDAR modulators and pharmaceutical effects on human neurons.