Description:
<jats:title>Abstract</jats:title>
<jats:p>Potato virus Y (PVY), a type member of the genus Potyvirus (family Potyviridae), is currently the most important virus infecting the potato crop. PVY is also a dangerous pathogen of the tomato, pepper, and tobacco. The reverse transcription loop-mediated amplification (RT-LAMP) is gaining recognition as a good alternative to RT-PCR in diagnosing plant viruses. Here, we provide a detailed description of a simple protocol for fast and sensitive detection of PVY by the RT-LAMP assay, which can be easily adapted to detect other plant pathogens, harboring both RNA and DNA genomes.</jats:p>