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Bahi, Muhammad; Jacob, Claus; Khairan, Khairan

EFEK SITOTOKSIK HAARLEM OIL TERHADAP HL-60 CELL LINE DAN Steinernema feltiae (Cytotoxic Effect of Haarlem Oil on HL-60 Cell Line and Steinernema feltiae)

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  • Media type: E-Article
  • Title: EFEK SITOTOKSIK HAARLEM OIL TERHADAP HL-60 CELL LINE DAN Steinernema feltiae (Cytotoxic Effect of Haarlem Oil on HL-60 Cell Line and Steinernema feltiae)
  • Contributor: Bahi, Muhammad; Jacob, Claus; Khairan, Khairan
  • Published: LPPM Unsyiah, 2016
  • Published in: Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences, 10 (2016) 2, Seite 110-114
  • Language: Not determined
  • DOI: 10.21157/j.ked.hewan.v10i2.5038
  • ISSN: 2502-5600; 1978-225X
  • Keywords: General Medicine
  • Origination:
  • Footnote:
  • Description: The objective of this research was to investigate cytotoxicity effect of Haarlem oil (HO) on HL-60 cell line and Steinernema feltiae (S. feltiae). The test results using trypan blue method and CellTiter-Glo® assays revealed that HO showed higher cytotoxicity effect against HL-60 cell line especially at concentration of 1:10 and 1:5. Meanwhile, the MTT (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay revealed that the higher cytotoxicity effect showed at 1:5, with the cell viabilities were around 40-65%. The nematicidal assay discovered that HO at concentration of 1:5 showed higher activities compare to other concentrations with percentage viability of 70%. These studies have shown that HO shows cytotoxic effect against HL-60 cell lines, and moderate activity against S. feltiae as well.