Description:
<jats:p>Gastric cancer is one of the most common cancers in the world. Delayed
diagnosis is the most common cause of death in patients. Long noncoding RNAs
(lncRNAs) are a group of non-coding RNAs that are effective in the incidence
of cancers. Studies in different cancers determined HOTAIR as an important
lncRNA in tumorigenesis. In gastric cancer, the function of HOTAIR in the
initiation and progression of cancer seems to be crucial. In this study, we
confirmed the significant differential expression of HOTAIR between gastric
tumors and normal tissues in different datasets. In the following, the
regulatory function of HOTAIR and its interaction with miRNAs in development
of gastric cancer was analyzed. Our analysis determined that the
upregulation of HOTAIR is essential to different pathways associated with
the progression of gastric cancer. Further analysis determined numerous
miRNAs as potential targets for HOTAIR. Among them, we demonstrated miR-1 as
a significant miRNA with negative correlation with HOTAIR in gastric tumors.
Validation analysis determined that HOTAIR is a target of cisplatin as a
common chemotherapy drug. Eventually, the effect of cisplatin on the
expression of HOTAIR and its potential target, miR-1, was checked by an in
vitro study. Cisplatin treatment on the gastric cancer cell line showed that
there is a significant negative correlation between the downregulation of
HOTAIR and the upregulation of miR-1 in treated cells. In conclusion,
comprehensive in silico analysis and experimental study provided evidence
for the importance of the HOTAIR/miR-1 axis as potential diagnostic and
treatment strategies for gastric cancer.</jats:p>