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Vivas, Monica Chavez; Villamarín-Guerrero, Héctor Fabio; Sanchez, Carlos Alberto

Interleukin-10 (IL-10) 1082 promoter polymorphisms and plasma IL-10 levels in patients with bacterial sepsis

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  • Media type: E-Article
  • Title: Interleukin-10 (IL-10) 1082 promoter polymorphisms and plasma IL-10 levels in patients with bacterial sepsis
  • Contributor: Vivas, Monica Chavez; Villamarín-Guerrero, Héctor Fabio; Sanchez, Carlos Alberto
  • imprint: Walter de Gruyter GmbH, 2021
  • Published in: Romanian Journal of Internal Medicine
  • Language: English
  • DOI: 10.2478/rjim-2020-0033
  • ISSN: 2501-062X
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title> <jats:p> <jats:bold>Background.</jats:bold> Interleukin-10 (IL-10) is a multifunctional cytokine which has been seen to play a relevant role in the pathogenesis of sepsis. We examined the association between a single nucleotide polymorphism (SNP) in IL-10-1082G/A in patients with sepsis in Cali city.</jats:p> <jats:p> <jats:bold>Methods.</jats:bold> A total of 100 patients with sepsis and 50 control subjects were enrolled in this study. Blood samples were collected from all patients in EDTA containing tubes. IL-10-1082G/A gene promoter polymorphism was analyzed by Sequence Specific Polymerase Chain Reaction (SS-PCR), while levels of serum IL-10 were measured by Enzyme Linked Immunoassay Assay (ELISA) in patients with sepsis and healthy controls.</jats:p> <jats:p> <jats:bold>Results.</jats:bold> AA homozygous genotype was found more frequently in patients (32%), compared with controls (18%). AA homozygous patients showed an increased risk of developing infection by Gram-negative bacteria (OR = 2,875; 95% CI = 1.162-7.113; p = 0.020), and significantly high plasma levels of IL-10 (OR = 4.800, 95% CI 1.652–13.944; p = 0.002). AA homozygous patients high plasma IL-10 levels have greater risk of developing sepsis (63.6%; OR = 4,894; 95% CI: 1,337–17,909; p = 0.002). In this group, Afro-Colombian individuals were overrepresented among the sepsis patients with high plasma IL-10 levels (OR = 1.661; 95% CI: 1.408–1.959; p = 0.036).</jats:p> <jats:p> <jats:bold>Conclusion.</jats:bold> Our study concluded that AA genotype of IL-10-1082G/A polymorphism is a risk factor for high IL-10 production and development of sepsis by Gram negative bacteria, especially in Afro-Colombian patients.</jats:p>
  • Access State: Open Access