Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum
You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Effects of Temperature, pH, and Agitation on Growth and Butanol Production of Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium saccharoperbutylacetonicum
Published in:Environmental and Climate Technologies
Language:
English
DOI:
10.2478/rtuect-2024-0007
ISSN:
2255-8837
Origination:
Footnote:
Description:
<jats:title>Abstract</jats:title>
<jats:p>Butanol is a promising alternative to fossil-derived fuels. <jats:italic>Clostridium</jats:italic> genus bacteria are known for their ability to produce butanol as one of the metabolites, however, at the moment this solution is not economically viable. To solve it, the process of butanol production should be optimized. While ABE fermentation has been extensively studied, information about the optimal growth conditions for specific microorganisms often differs from one study to another. Therefore, this study aims to search for optimal growth conditions in sealed serum bottle tests for three widely used strains in ABE fermentation. In this study effects of temperature, pH, and agitation were tested on <jats:italic>Clostridium acetobutylicum</jats:italic>, <jats:italic>Clostridium beijerinckii</jats:italic>, and <jats:italic>Clostridium saccharoperbutylacetonicum</jats:italic>. The optimal temperature for <jats:italic>C. beijerinckii</jats:italic> growth and butanol production was 32 °C, the optimal agitation speed for growth was 0 rpm, but for butanol production, it was 200 rpm. For <jats:italic>C. saccharoperbutylacetonicum</jats:italic> growth and butanol production pH 7.5, 30 °C temperature and an agitation rate of 100 rpm were optimal, however, this effect was slight. For <jats:italic>C. acetobutylicum</jats:italic> cultivation optimal temperature, pH, and agitation rate were respectively 37 °C, 6.5, and 200 rpm.</jats:p>