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Holl, Myriam; Rasch, Marie-Lena; Becker, Lucas; Keller, Anna-Lena; Schultze-Rhonhof, Laura; Ruoff, Felix; Templin, Markus; Keller, Silke; Neis, Felix; Keßler, Franziska; Andress, Jürgen; Bachmann, Cornelia; Krämer, Bernhard; Schenke-Layland, Katja; Brucker, Sara; Marzi, Julia; Weiss, Martin

Cell Type-Specific Anti-Adhesion Properties of Peritoneal Cell Treatment with Plasma-Activated Media (PAM)

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  • Media type: E-Article
  • Title: Cell Type-Specific Anti-Adhesion Properties of Peritoneal Cell Treatment with Plasma-Activated Media (PAM)
  • Contributor: Holl, Myriam; Rasch, Marie-Lena; Becker, Lucas; Keller, Anna-Lena; Schultze-Rhonhof, Laura; Ruoff, Felix; Templin, Markus; Keller, Silke; Neis, Felix; Keßler, Franziska; Andress, Jürgen; Bachmann, Cornelia; Krämer, Bernhard; Schenke-Layland, Katja; Brucker, Sara; Marzi, Julia; Weiss, Martin
  • imprint: MDPI AG, 2022
  • Published in: Biomedicines
  • Language: English
  • DOI: 10.3390/biomedicines10040927
  • ISSN: 2227-9059
  • Keywords: General Biochemistry, Genetics and Molecular Biology ; Medicine (miscellaneous)
  • Origination:
  • Footnote:
  • Description: <jats:p>Postoperative abdominal adhesions are responsible for serious clinical disorders. Administration of plasma-activated media (PAM) to cell type-specific modulated proliferation and protein biosynthesis is a promising therapeutic strategy to prevent pathological cell responses in the context of wound healing disorders. We analyzed PAM as a therapeutic option based on cell type-specific anti-adhesive responses. Primary human peritoneal fibroblasts and mesothelial cells were isolated, characterized and exposed to different PAM dosages. Cell type-specific PAM effects on different cell components were identified by contact- and marker-independent Raman imaging, followed by thorough validation by specific molecular biological methods. The investigation revealed cell type-specific molecular responses after PAM treatment, including significant cell growth retardation in peritoneal fibroblasts due to transient DNA damage, cell cycle arrest and apoptosis. We identified a therapeutic dose window wherein specifically pro-adhesive peritoneal fibroblasts were targeted, whereas peritoneal mesothelial cells retained their anti-adhesive potential of epithelial wound closure. Finally, we demonstrate that PAM treatment of peritoneal fibroblasts reduced the expression and secretion of pro-adhesive cytokines and extracellular matrix proteins. Altogether, we provide insights into biochemical PAM mechanisms which lead to cell type-specific pro-therapeutic cell responses. This may open the door for the prevention of pro-adhesive clinical disorders.</jats:p>
  • Access State: Open Access