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Media type:
E-Article
Title:
Evaluation of the Effects of Different Sample Collection Strategies on DNA/RNA Co-Analysis of Forensic Stains
Contributor:
Lacerenza, Daniela;
Caudullo, Giorgio;
Chierto, Elena;
Aneli, Serena;
Di Vella, Giancarlo;
Barberis, Marco;
Voyron, Samuele;
Berchialla, Paola;
Robino, Carlo
Published:
MDPI AG, 2022
Published in:
Genes, 13 (2022) 6, Seite 983
Language:
English
DOI:
10.3390/genes13060983
ISSN:
2073-4425
Origination:
Footnote:
Description:
<jats:p>The aim of this study was to evaluate the impact of different moistening agents (RNase-free water, absolute anhydrous ethanol, RNAlater®) applied to collection swabs on DNA/RNA retrieval and integrity for capillary electrophoresis applications (STR typing, cell type identification by mRNA profiling). Analyses were conducted on whole blood, luminol-treated diluted blood, saliva, semen, and mock skin stains. The effects of swab storage temperature and the time interval between sample collection and DNA/RNA extraction were also investigated. Water provided significantly higher DNA yields than ethanol in whole blood and semen samples, while ethanol and RNAlater® significantly outperformed water in skin samples, with full STR profiles obtained from over 98% of the skin samples collected with either ethanol or RNAlater®, compared to 71% of those collected with water. A significant difference in mRNA profiling success rates was observed in whole blood samples between swabs treated with either ethanol or RNAlater® (100%) and water (37.5%). Longer swab storage times before processing significantly affected mRNA profiling in saliva stains, with the success rate decreasing from 91.7% after 1 day of storage to 25% after 7 days. These results may contribute to the future development of optimal procedures for the collection of different types of biological traces.</jats:p>