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Lobo de Sá, Fábia Daniela; Butkevych, Eduard; Nattramilarasu, Praveen Kumar; Fromm, Anja; Mousavi, Soraya; Moos, Verena; Golz, Julia C.; Stingl, Kerstin; Kittler, Sophie; Seinige, Diana; Kehrenberg, Corinna; Heimesaat, Markus M.; Bereswill, Stefan; Schulzke, Jörg-Dieter; Bücker, Roland

Curcumin Mitigates Immune-Induced Epithelial Barrier Dysfunction by Campylobacter jejuni

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  • Media type: E-Article
  • Title: Curcumin Mitigates Immune-Induced Epithelial Barrier Dysfunction by Campylobacter jejuni
  • Contributor: Lobo de Sá, Fábia Daniela; Butkevych, Eduard; Nattramilarasu, Praveen Kumar; Fromm, Anja; Mousavi, Soraya; Moos, Verena; Golz, Julia C.; Stingl, Kerstin; Kittler, Sophie; Seinige, Diana; Kehrenberg, Corinna; Heimesaat, Markus M.; Bereswill, Stefan; Schulzke, Jörg-Dieter; Bücker, Roland
  • Published: MDPI AG, 2019
  • Published in: International Journal of Molecular Sciences, 20 (2019) 19, Seite 4830
  • Language: English
  • DOI: 10.3390/ijms20194830
  • ISSN: 1422-0067
  • Keywords: Inorganic Chemistry ; Organic Chemistry ; Physical and Theoretical Chemistry ; Computer Science Applications ; Spectroscopy ; Molecular Biology ; General Medicine ; Catalysis
  • Origination:
  • Footnote:
  • Description: <jats:p>Campylobacter jejuni (C. jejuni) is the most common cause of foodborne gastroenteritis worldwide. The bacteria induce diarrhea and inflammation by invading the intestinal epithelium. Curcumin is a natural polyphenol from turmeric rhizome of Curcuma longa, a medical plant, and is commonly used in curry powder. The aim of this study was the investigation of the protective effects of curcumin against immune-induced epithelial barrier dysfunction in C. jejuni infection. The indirect C. jejuni-induced barrier defects and its protection by curcumin were analyzed in co-cultures with HT-29/B6-GR/MR epithelial cells together with differentiated THP-1 immune cells. Electrophysiological measurements revealed a reduction in transepithelial electrical resistance (TER) in infected co-cultures. An increase in fluorescein (332 Da) permeability in co-cultures as well as in the germ-free IL-10−/− mouse model after C. jejuni infection was shown. Curcumin treatment attenuated the C. jejuni-induced increase in fluorescein permeability in both models. Moreover, apoptosis induction, tight junction redistribution, and an increased inflammatory response—represented by TNF-α, IL-1β, and IL-6 secretion—was observed in co-cultures after infection and reversed by curcumin. In conclusion, curcumin protects against indirect C. jejuni-triggered immune-induced barrier defects and might be a therapeutic and protective agent in patients.</jats:p>
  • Access State: Open Access