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Di Gianfrancesco, Luca; Montagner, Isabella Monia; Tormen, Debora; Crestani, Alessandro; Amodeo, Antonio; Corsi, Paolo; De Marchi, Davide; Miglioranza, Eugenio; Lista, Giuliana; Simonetti, Francesca; Busetto, Gian Maria; Maggi, Martina; Marino, Filippo; Scapinello, Antonio; Porreca, Angelo

The Feasibility and Diagnostic Adequacy of PD-L1 Expression Analysis Using the Cytoinclusion Technique in Bladder Cancer: A Prospective Single-Center Study

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  • Media type: E-Article
  • Title: The Feasibility and Diagnostic Adequacy of PD-L1 Expression Analysis Using the Cytoinclusion Technique in Bladder Cancer: A Prospective Single-Center Study
  • Contributor: Di Gianfrancesco, Luca; Montagner, Isabella Monia; Tormen, Debora; Crestani, Alessandro; Amodeo, Antonio; Corsi, Paolo; De Marchi, Davide; Miglioranza, Eugenio; Lista, Giuliana; Simonetti, Francesca; Busetto, Gian Maria; Maggi, Martina; Marino, Filippo; Scapinello, Antonio; Porreca, Angelo
  • Published: MDPI AG, 2024
  • Published in: Journal of Clinical Medicine, 13 (2024) 14, Seite 4072
  • Language: English
  • DOI: 10.3390/jcm13144072
  • ISSN: 2077-0383
  • Origination:
  • Footnote:
  • Description: Background: Programmed death-ligand 1 (PD-L1) expression has been recognized as a potential biomarker for various cancers, yet its diagnostic and prognostic significance in urothelial bladder cancer (BCa) requires further investigation. Methods: In this prospective single-center study, we aimed to assess the feasibility and diagnostic adequacy of PD-L1 expression analysis using cytoinclusion in BCa patients. We enrolled consecutive patients undergoing endoscopic transurethral resection of bladder tumor (TURBT), repeat TURBT, or robot-assisted radical cystectomy. Urinary and tissue specimens were collected from these patients for cytoinclusion and histopathological analysis to evaluate PD-L1 expression. Results: Out of 29 patients, PD-L1 expression was detected from cytoinclusion in 42.8% (3 out of 7), 10% (1 out of 10), and 66.8% (8 out of 12) of patients with negative/papilloma, low-grade, and high-grade tumors, respectively. Conversely, histopathological analysis identified PD-L1 expression in 57.2% (4 out of 7), 30% (3 out of 10), and 83.3% (10 out of 12) of patients with negative/papilloma, low-grade, and high-grade tumors, respectively. The diagnostic concordance between cytoinclusion and histopathology was 85.7%, 80%, and 83.3% in patients with negative/papilloma, low-grade, and high-grade tumors, respectively. Conclusions: Our study underscores the promise of cytoinclusion as a minimally invasive method for quantifying urinary PD-L1 percentages. This approach could serve as both a potential prognostic and diagnostic indicator, easily obtainable from urine samples. Standardizing this technique could facilitate its widespread use as a valuable tool.
  • Access State: Open Access