Khursheed, Anjum;
Ahmad, Saeed;
Khan, Kashif-ur-Rehman;
Tousif, Muhammad Imran;
Aati, Hanan Y.;
Ovatlarnporn, Chitchamai;
Rao, Huma;
Khurshid, Umair;
Ghalloo, Bilal Ahmad;
Tabassum, Sobia;
Basit, Abdul
Efficacy of Phytochemicals Derived from Roots of Rondeletia odorata as Antioxidant, Antiulcer, Diuretic, Skin Brightening and Hemolytic Agents—A Comprehensive Biochemical and In Silico Study
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Media type:
E-Article
Title:
Efficacy of Phytochemicals Derived from Roots of Rondeletia odorata as Antioxidant, Antiulcer, Diuretic, Skin Brightening and Hemolytic Agents—A Comprehensive Biochemical and In Silico Study
Contributor:
Khursheed, Anjum;
Ahmad, Saeed;
Khan, Kashif-ur-Rehman;
Tousif, Muhammad Imran;
Aati, Hanan Y.;
Ovatlarnporn, Chitchamai;
Rao, Huma;
Khurshid, Umair;
Ghalloo, Bilal Ahmad;
Tabassum, Sobia;
Basit, Abdul
imprint:
MDPI AG, 2022
Published in:Molecules
Language:
English
DOI:
10.3390/molecules27134204
ISSN:
1420-3049
Origination:
Footnote:
Description:
<jats:p>Roots of Rondeletia odorata are a rich source of phytochemicals with high antioxidant potential and thus may possess health benefits. This study used the LC-MS technique to identify phytoconstituents in R. odorata roots extract/fractions. Results revealed that n-butanol fraction and ethanolic extract contained total phenolic and flavonoid contents with values of 155.64 ± 0.66 mgGAE/g DE and 194.94 ± 0.98 mgQE/g DE, respectively. Significant potential of antioxidants was observed by DPPH, CUPRAC and FRAP methods while the ABTS method showed moderate antioxidant potential. Maximum % inhibition for urease, tyrosinase and carbonic anhydrase was shown by ethanolic extract (73.39 ± 1.11%), n-butanol soluble fraction (80.26 ± 1.59%) and ethyl acetate soluble fraction (76.50 ± 0.67%) which were comparable with thiourea (standard) (98.07 ± 0.74%), kojic acid (standard) (98.59 ± 0.92%) and acetazolamide (standard) (95.51 ± 1.29%), respectively, while all other extract/fractions showed moderate inhibition activity against these three enzymes. Hemolytic activity was also observed to range from 18.80 ± 0.42 to 3.48 ± 0.69% using the standard (triton X-100) method. In total, 28 and 20 compounds were identified tentatively by LC-MS analysis of ethanolic extract and n-butanol soluble fraction, respectively. Furthermore, molecular docking was undertaken for major compounds identified by LC-MS for determining binding affinity between enzymes (urease, tyrosinase and carbonic anhydrase) and ligands. It was concluded that active phytochemicals were present in roots of R. odorata with potential for multiple pharmacological applications and as a latent source of pharmaceutically important compounds. This should be further explored to isolate important constituents that could be used in treating different diseases.</jats:p>