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Thurner, Lorenz; Fadle, Natalie; Regitz, Evi; Roth, Sophie; Cetin, Onur; Kos, Igor Age; Hess, Simon Mauro; Bein, Julia; Bohle, Rainer Maria; Vornanen, Martine; Sundström, Christer; De Leval, Laurence; Tiacci, Enrico; Borchmann, Peter; Engert, Andreas; Poeschel, Viola; Held, Gerhard; Schwarz, Eva C.; Neumann, Frank; Preuss, Klaus-Dieter; Hoth, Markus; Küppers, Ralf; Lehman, Karola; Hansmann, Martin-Leo; [...]

B-cell receptor reactivity against Rothia mucilaginosa in nodular lymphocyte-predominant Hodgkin lymphoma

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  • Media type: E-Article
  • Title: B-cell receptor reactivity against Rothia mucilaginosa in nodular lymphocyte-predominant Hodgkin lymphoma
  • Contributor: Thurner, Lorenz; Fadle, Natalie; Regitz, Evi; Roth, Sophie; Cetin, Onur; Kos, Igor Age; Hess, Simon Mauro; Bein, Julia; Bohle, Rainer Maria; Vornanen, Martine; Sundström, Christer; De Leval, Laurence; Tiacci, Enrico; Borchmann, Peter; Engert, Andreas; Poeschel, Viola; Held, Gerhard; Schwarz, Eva C.; Neumann, Frank; Preuss, Klaus-Dieter; Hoth, Markus; Küppers, Ralf; Lehman, Karola; Hansmann, Martin-Leo; [...]
  • imprint: Ferrata Storti Foundation (Haematologica), 2023
  • Published in: Haematologica
  • Language: Not determined
  • DOI: 10.3324/haematol.2023.282698
  • ISSN: 1592-8721; 0390-6078
  • Keywords: Hematology
  • Origination:
  • Footnote:
  • Description: <jats:p>Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is a Hodgkin lymphoma expressing functional B-cell receptors (BCR). Recently, we described a dual stimulation model of IgD+ lymphocyte-predominant cells by Moraxella catarrhalis antigen RpoC and its superantigen MID/hag, associated with extralong CDR3 and HLA-DRB1*04 or HLADRB1* 07 haplotype. The aim of the present study was to extend the antigen screening to further bacteria and viruses. The fragment antibody-binding (Fab) regions of seven new and 15 previously reported cases were analyzed. The reactivity of non-Moraxella spp.-reactive Fab regions against lysates of Rothia mucilaginosa was observed in 5/22 (22.7%) cases. Galactofuranosyl transferase (Gltf) and 2,3-butanediol dehydrogenase (Bdh) of R. mucilaginosa were identified by comparative silver- and immuno-staining in two-dimensional gels, with subsequent mass spectrometry and validation by western blots and enzyme-linked immunosorbent assay. Both R. mucilaginosa Gltf and Bdh induced BCR pathway activation and proliferation in vitro. Apoptosis was induced by recombinant Gltf/ETA’-immunotoxin conjugates in DEV cells expressing recombinant R. mucilaginosa-reactive BCR. Reactivity against M. catarrhalis RpoC was confirmed in 3/7 newly expressed BCR (total 10/22 reactive to Moraxella spp.), resulting in 15/22 (68.2%) cases with BCR reactivity against defined bacterial antigens. These findings strengthen the hypothesis of bacterial trigger contributing to subsets of NLPHL.</jats:p>
  • Access State: Open Access