Fanning, Laura R.;
Finney, Marcie R.;
Winter, Daniel G.;
Greco, Nicholas J.;
Kozik, Margaret M.;
Laughlin, Mary J.
Human umbilical cord blood (UCB) derived CD133 cells demonstrate homing capability in vasculogenesis and evoke a proliferative response as well as IL-4 and IL-10 cytokine production in vitro (102.3)
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Media type:
E-Article
Title:
Human umbilical cord blood (UCB) derived CD133 cells demonstrate homing capability in vasculogenesis and evoke a proliferative response as well as IL-4 and IL-10 cytokine production in vitro (102.3)
Contributor:
Fanning, Laura R.;
Finney, Marcie R.;
Winter, Daniel G.;
Greco, Nicholas J.;
Kozik, Margaret M.;
Laughlin, Mary J.
imprint:
The American Association of Immunologists, 2007
Description:
<jats:title>Abstract</jats:title>
<jats:p>Homing, IL-8 production and immunogenicity are aspects of CD133 cells, a population of stem cells, which may augment postnatal angiogenesis therapy. CD133 cells were isolated from UCB mononuclear cells (MNC) by magnetic bead selection (Miltenyi) and analyzed by flow cytometry. CD133 cells showed co-expression of KDR (3.5%), CD105 (22.7%), CXCR4 (8.7%), HLA-DR (57.6%) and HLA-ABC (66.5%), suggesting CD133 cells are capable of presenting alloantigen. We next performed 3H-thymidine incorporation MLR using adult peripheral blood (AB) MNC stimulated by irradiated UCB CD133 (46939 ± 2764 cpm) and MNC (49548 ± 2018 cpm). MLR studies with CSFE-stained responder cells confirmed equivalent rates of cell division. The observed proliferation was not an artifact of CD133 cell selection. Preliminary comparison studies of cytokine production of IL-4 and IL-10 increased when AB MNC were stimulated by CD133 as compared to UCB MNC (IL-4: 141±45.7pg/mL and IL-10: 964±538pg/mL,). Additionally, UCB CD133 production of IL-8 was elevated as compared to MNC (103±380 pg/mL). Transwell migration to SDF-1 (100ng/mL) demonstrated a 1.8 ± 0.7 fold increase compared to basal media, correlating with CXCR4 expression. In summary, UCB CD133 cells demonstrate homing capability, potential cellular recruitment (IL-8 production) demonstrate TH2 skewing and induce AB MNC proliferation relevant to angiogenesis and cellular therapeutics.</jats:p>