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Media type:
E-Article
Title:
ERG-28 controls BK channel trafficking in the ER to regulate synaptic function and alcohol response in C. elegans
Contributor:
Oh, Kelly H;
Haney, James J;
Wang, Xiaohong;
Chuang, Chiou-Fen;
Richmond, Janet E;
Kim, Hongkyun
Published:
eLife Sciences Publications, Ltd, 2017
Published in:
eLife, 6 (2017)
Language:
English
DOI:
10.7554/elife.24733
ISSN:
2050-084X
Origination:
Footnote:
Description:
Voltage- and calcium-dependent BK channels regulate calcium-dependent cellular events such as neurotransmitter release by limiting calcium influx. Their plasma membrane abundance is an important factor in determining BK current and thus regulation of calcium-dependent events. In C. elegans, we show that ERG-28, an endoplasmic reticulum (ER) membrane protein, promotes the trafficking of SLO-1 BK channels from the ER to the plasma membrane by shielding them from premature degradation. In the absence of ERG-28, SLO-1 channels undergo aspartic protease DDI-1-dependent degradation, resulting in markedly reduced expression at presynaptic terminals. Loss of erg-28 suppressed phenotypic defects of slo-1 gain-of-function mutants in locomotion, neurotransmitter release, and calcium-mediated asymmetric differentiation of the AWC olfactory neuron pair, and conferred significant ethanol-resistant locomotory behavior, resembling slo-1 loss-of-function mutants, albeit to a lesser extent. Our study thus indicates that the control of BK channel trafficking is a critical regulatory mechanism for synaptic transmission and neural function.