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Jean, Philippe; Lopez de la Morena, David; Michanski, Susann; Jaime Tobón, Lina María; Chakrabarti, Rituparna; Picher, Maria Magdalena; Neef, Jakob; Jung, SangYong; Gültas, Mehmet; Maxeiner, Stephan; Neef, Andreas; Wichmann, Carolin; Strenzke, Nicola; Grabner, Chad; Moser, Tobias

The synaptic ribbon is critical for sound encoding at high rates and with temporal precision

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  • Media type: E-Article
  • Title: The synaptic ribbon is critical for sound encoding at high rates and with temporal precision
  • Contributor: Jean, Philippe; Lopez de la Morena, David; Michanski, Susann; Jaime Tobón, Lina María; Chakrabarti, Rituparna; Picher, Maria Magdalena; Neef, Jakob; Jung, SangYong; Gültas, Mehmet; Maxeiner, Stephan; Neef, Andreas; Wichmann, Carolin; Strenzke, Nicola; Grabner, Chad; Moser, Tobias
  • Published: eLife Sciences Publications, Ltd, 2018
  • Published in: eLife, 7 (2018)
  • Language: English
  • DOI: 10.7554/elife.29275
  • ISSN: 2050-084X
  • Origination:
  • Footnote:
  • Description: We studied the role of the synaptic ribbon for sound encoding at the synapses between inner hair cells (IHCs) and spiral ganglion neurons (SGNs) in mice lacking RIBEYE (RBEKO/KO). Electron and immunofluorescence microscopy revealed a lack of synaptic ribbons and an assembly of several small active zones (AZs) at each synaptic contact. Spontaneous and sound-evoked firing rates of SGNs and their compound action potential were reduced, indicating impaired transmission at ribbonless IHC-SGN synapses. The temporal precision of sound encoding was impaired and the recovery of SGN-firing from adaptation indicated slowed synaptic vesicle (SV) replenishment. Activation of Ca2+-channels was shifted to more depolarized potentials and exocytosis was reduced for weak depolarizations. Presynaptic Ca2+-signals showed a broader spread, compatible with the altered Ca2+-channel clustering observed by super-resolution immunofluorescence microscopy. We postulate that RIBEYE disruption is partially compensated by multi-AZ organization. The remaining synaptic deficit indicates ribbon function in SV-replenishment and Ca2+-channel regulation.
  • Access State: Open Access