You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains
Contributor:
Beatty, Kimberly E.;
Williams, Monique;
Carlson, Brian L.;
Swarts, Benjamin M.;
Warren, Robin M.;
van Helden, Paul D.;
Bertozzi, Carolyn R.
imprint:
National Academy of Sciences, 2013
Published in:Proceedings of the National Academy of Sciences of the United States of America
Language:
English
ISSN:
0027-8424
Origination:
Footnote:
Description:
<p>Most current diagnostic tests for tuberculosis do not reveal the species or strain of pathogen causing pulmonary infection, which can lead to inappropriate treatment regimens and the spread of disease. Here, we report an assay for mycobacterial strain assignment based on genetically conserved mycobacterial sulfatases. We developed a sulfatase-activated probe, 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid detection of sulfatases in mycobacterial lysates. This assay revealed that mycobacterial strains have distinct sulfatase fingerprints that can be used to judge both the species and lineage. Our results demonstrate the potential of enzyme-activated probes for rapid pathogen discrimination for infectious diseases.</p>