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Media type:
E-Article
Title:
Demonstration of Globin Messenger Sequences in Giant Nuclear Precursors of Messenger RNA of Avian Erythroblasts
Contributor:
Imaizumi, Tereza;
Diggelmann, Heidi;
Scherrer, Klaus
imprint:
National Academy of Sciences of the United States of America, 1973
Published in:Proceedings of the National Academy of Sciences of the United States of America
Language:
English
ISSN:
0027-8424
Origination:
Footnote:
Description:
<p>Highly purified globin mRNA from ducks was copied with RNA-directed DNA polymerase from avian myeloblastosis virus into anti-messenger DNA. With excess RNA, more than 90% of this DNA annealed back to its template with a C<sub>o</sub>t/2 value of 7.5 × 10<sup>-4</sup>mol· sec· liter<sup>-1</sup>; the melting temperature of the hybrid was 86 degrees. Giant nuclear RNA fractions with sedimentation coefficients of more than 50 S formed hybrids of almost equal stability at C<sub>o</sub>t/2 values of 0.05-0.42 mol· sec· liter<sup>-1</sup>, indicating amRNA content of 0.3-1.5%. 12S RNA from the same polyribosomes and nuclear giant RNA from HeLa cells did not cross-hybridize. Although a large part of the giant RNA broke down in 99% dimethylsulfoxide gradients, RNA fractions sedimenting faster than 28S rRNA still were found to consist of up to 0.03% globin mRNA sequences. Thus, the mRNA sequences are contained in the covalent structure of giant nuclear precursors, which are termed precursor-mRNA.</p>