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Media type:
E-Article
Title:
Chromosomal Locations of Mouse Immunoglobulin Genes
Contributor:
Valbuena, Oscar;
Marcu, Kenneth B.;
Croce, Carlo M.;
Huebner, Kay;
Weigert, Martin;
Perry, Robert P.
imprint:
National Academy of Sciences of the United States of America, 1978
Published in:Proceedings of the National Academy of Sciences of the United States of America
Language:
English
ISSN:
0027-8424
Origination:
Footnote:
Description:
<p>The chromosomal locations of the structural genes coding for the constant portions of mouse heavy (H) and light chain immunoglobulins were studied by molecular hybridization techniques. Complementary DNA probes containing the constant-region sequences of k and λ<sub>I</sub>light chain and α , γ<sub>2b</sub>, and μ heavy chain mRNAs were annealed to a large excess of DNA from a series of eight mouse-human hybrid cell lines that are deficient for various mouse chromosomes. The lines were scored as positive when a high proportion of a probe annealed and negative when an insignificant proportion annealed. Some lines were clearly negative for H and λ and clearly positive for k. Others were positive or intermediate for λ , positive for k and negative for H. Still others, including a line that was selected for the absence of the mouse X chromosome, were positive for all immunoglobulin species. These results demonstrate that the C<sub>λ</sub>, C<sub>κ</sub>, and C<sub>H</sub>genes are located on different autosomes in the mouse. In contrast, the three heavy-chain families exhibited consistently uniform hybridization results, suggesting that the genes for C<sub>α</sub>, C<sub>γ</sub>, and C<sub>μ</sub>are located on the same chromosome. A comparison of karyotypic data with hybridization data has limited the possible locations of the Ig genes to only a few chromosomes.</p>