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Media type:
E-Article
Title:
A Genetic Approach to Analyzing Membrane Protein Topology
Contributor:
Manoil, Colin;
Beckwith, Jon
Published:
The American Association for the Advancement of Science, 1986
Published in:
Science, 233 (1986) 4771, Seite 1403-1408
Language:
English
ISSN:
0036-8075;
1095-9203
Origination:
Footnote:
Description:
<p>Fusions of the secreted protein alkaline phosphatase to an integral cytoplasmic membrane protein of Escherichia coli showed different activities depending on where in the membrane protein the alkaline phosphatase was fused. Fusions to positions in or near the periplasmic domain led to high alkaline phosphatase activity, whereas those to positions in the cytoplasmic domain gave low activity. Analysis of alkaline phosphatase fusions to membrane proteins of unknown structure may thus be generally useful in determining their membrane topologies.</p>