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Media type:
E-Article
Title:
Transdominant Inhibition of Tyrosine Kinase Activity in Mutant Insulin/ Insulin-Like Growth Factor I Hybrid Receptors
Contributor:
Treadway, Judith L.;
Morrison, Brian D.;
Soos, Maria A.;
Siddle, Kenneth;
Olefsky, Jerrold;
Ullrich, Axel;
McClain, Donald A.;
Pessin, Jeffrey E.
Published:
National Academy of Sciences of the United States of America, 1991
Published in:
Proceedings of the National Academy of Sciences of the United States of America, 88 (1991) 1, Seite 214-218
Language:
English
ISSN:
0027-8424
Origination:
Footnote:
Description:
Classical insulin and insulin-like growth factor I (IGF-I) receptors exist as well defined α2β2heterotetrameric complexes that are assembled from two identical αβ heterodimeric half-receptor precursors. Recent evidence suggests that insulin and IGF-I half-receptors can heterologously assemble to form α2β2insulin/IGF-I hybrid receptor complexes in vivo and in vitro. We have utilized hybrid receptor complexes to examine ligand-stimulated transmembrane signaling of wild-type insulin (αβINS.WT) or IGF-I (αβIGF.WT) half-receptors assembled with a kinase-defective insulin half-receptor mutant (αβINS.A/K). In vitro assembly of either (αβ)IGF.WT/(αβ)INS.A/Kor (αβ)INS.WT/(αβ)INS.A/Khybrid receptors resulted in decreased substrate protein kinase activity. The degree of protein kinase inactivation directly correlated with the amount of immunologically cross-reactive hybrid receptors formed. In contrast to substrate kinase activity, insulin-stimulated autophosphorylation of the (αβ)INS.WT/(αβ)INS.A/Khybrid receptor complex was completely unaffected in comparison to the wild-type (αβ)INS.WT/(αβ)INS.WTreceptor. To assess a molecular basis for this difference, autophosphorylation of a hybrid receptor composed of a truncated β-subunit insulin half-receptor with the kinase-defective half-receptor, (αβ)INS.Δ CT/(αβ)INS.A/K, demonstrated the exclusive autophosphorylation of the (αβ)INS.A/Khalf-receptor β subunit. These results demonstrate that ligand-dependent substrate phosphorylation by insulin and IGF-I holoreceptors requires interactions between two functional β subunits within the α2β2heterotetrameric complex and occurs through an intramolecular trans-phosphorylation reaction.