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Media type:
E-Article
Title:
Control of Trehalase Synthesis in Neurospora crassa
Contributor:
Hanks, David L.;
Sussman, A. S.
Published:
American Botanical Society, 1969
Published in:
American Journal of Botany, 56 (1969) 10, Seite 1160-1166
Language:
English
ISSN:
1537-2197;
0002-9122
Origination:
Footnote:
Description:
<p>When an aconidial strain (STL6A) of Neurospora crassa is grown on carbon sources such as glucose, maltose, sucrose, etc., trehalase activity per unit weight of mycelium is very low. By contrast, media containing arabinose, glutamic acid, glycine, etc., which support growth only poorly, produce mycelium with very high trehalase activity. Retarding growth limiting the supply of a necessary nutrient, altering the pH and temperature, or adding toxic substances, however, does not derepress trehalase activity. Repression and derepression of trehalase was found to be reversible through the transfer of cultures to appropriate media. It is likely that the increase in trehalase activity results from de novo synthesis because labeled enzyme can be isolated from acrylamide gels after isolation from medium containing C<sup>14</sup>-labeled leucine and after purification by other means. These experiments are interpreted in terms of catabolite repression which may be correlated with events during growth and conidiation.</p>