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Media type:
E-Article
Title:
Troy/TNFRSF19 marks epithelial progenitor cells during mouse kidney development that continue to contribute to turnover in adult kidney
Contributor:
Schutgens, Frans;
Rookmaaker, Maarten B.;
Blokzijl, Francis;
van Boxtel, Ruben;
Vries, Robert;
Cuppen, Edwin;
Verhaar, Marianne C.;
Clevers, Hans
Published:
National Academy of Sciences, 2017
Published in:
Proceedings of the National Academy of Sciences of the United States of America, 114 (2017) 52, Seite E11190-E11198
Language:
English
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
During kidney development, progressively committed progenitor cells give rise to the distinct segments of the nephron, the functional unit of the kidney. Similar segment-committed progenitor cells are thought to be involved in the homeostasis of adult kidney. However, markers for most segment-committed progenitor cells remain to be identified. Here, we evaluate Troy/TNFRSF19 as a segment-committed nephron progenitor cell marker. Troy is expressed in the ureteric bud during embryonic development. During postnatal nephrogenesis, Troy⁺ cells are present in the cortex and papilla and display an immature tubular phenotype. Tracing of Troy⁺ cells during nephrogenesis demonstrates that Troy⁺ cells clonally give rise to tubular structures that persist for up to 2 y after induction. Troy⁺ cells have a 40-fold higher capacity than Troy⁻ cells to form organoids, which is considered a stem cell property in vitro. In the adult kidney, Troy⁺ cells are present in the papilla and these cells continue to contribute to collecting duct formation during homeostasis. The number of Troy-derived cells increases after folic acid-induced injury. Our data show that Troy marks a renal stem/progenitor cell population in the developing kidney that in adult kidney contributes to homeostasis, predominantly of the collecting duct, and regeneration.