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Media type:
E-Article
Title:
The Differentiation of Bacteria of the Colonaerogenes Family by the Use of Indicators
Contributor:
Clark, William Mansfield;
Lubs, Herbert A.
Published:
Memorial Institute for Infectious Diseases, 1915
Published in:
The Journal of Infectious Diseases, 17 (1915) 1, Seite 160-173
Language:
English
ISSN:
0022-1899
Origination:
Footnote:
Description:
<p>The diagnostic value of the gas ratio and the sanitary significance of the correlation between gas ratios and the sources of the organisms of the colon-aerogenes family studied in this laboratory have suggested the utility of a simple test which will distinguish the two main groups. By the use of the hydrogen electrode, the changes in hydrogen ion concentration have been followed in various cultures of the bacteria in question. By such studies differences in the metabolism of different species have been detected, and the correlation of these differences with the gas ratios has been established. Simple conditions have been found under which metabolism can be so controlled that the hydrogen ion concentrations of cultures of one group can be made to diverge widely from those of the other group. These differences can be sharply distinguished by means of paranitrophenol or methyl red. In this way a simple diagnostic test has been established the results of which correlate perfectly with the gas ratios of the two ain groups of the colon-aerogenes bacteria.</p>