Effect of pH on Low-Density Lipoprotein Oxidation by <tex-math>${\rm O}_{2}^{\overline{\cdot}}/{\rm HO}_{2}^{\bullet}$</tex-math> Free Radicals Produced by Gamma Radiolysis
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Media type:
E-Article
Title:
Effect of pH on Low-Density Lipoprotein Oxidation by <tex-math>${\rm O}_{2}^{\overline{\cdot}}/{\rm HO}_{2}^{\bullet}$</tex-math> Free Radicals Produced by Gamma Radiolysis
Description:
This study aimed to analyze quantitatively the initiation and the consequences of low-density lipoprotein (LDL) peroxidation by <tex-math>${\rm O}_{2}^{\overline{\cdot}}/{\rm HO}_{2}^{\bullet}$</tex-math> free radicals produced by gamma radiolysis. The action of increasing radiation doses on aqueous LDL solutions has been monitored simultaneously by several parameters: a decrease in endogenous vitamin E, the formation of thiobarbituric acid-reactive substances (TBARS) and conjugated dienes, the appearance of a differential fluorescence (excitation wavelength = 360 nm), and an increase of the relative electrophoretic mobility. Initial radiation yields (decrease in vitamin E, formation of TBARS) have been determined at pH 7 and pH 5.7 as a function of LDL concentration (from 0.75 to 9 g liter<sup>-1</sup>). From the comparison of these yields with those of <tex-math>${\rm O}_{2}^{\overline{\cdot}}$</tex-math> radicals produced by water radiolysis, we have deduced reaction mechanisms for LDL peroxidation initiated by <tex-math>${\rm O}_{2}^{\overline{\cdot}}/{\rm HO}_{2}^{\bullet}$</tex-math> free radicals.