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Media type:
E-Article
Title:
In vitro Fertilization with Isolated, Single Gametes Results in Zygotic Embryogenesis and Fertile Maize Plants
Contributor:
Kranz, Erhard;
Lörz, Horst
Published:
American Society of Plant Physiologists, 1993
Published in:
The Plant Cell, 5 (1993) 7, Seite 739-746
Language:
English
ISSN:
1040-4651
Origination:
Footnote:
Description:
We demonstrate here the possibility of regenerating phenotypically normal, fertile maize plants via in vitro fertilization of isolated, single sperm and egg cells mediated by electrofusion. The technique leads to the highly efficient formation of polar zygotes, globular structures, proembryos, and transition-phase embryos and to the formation of plants from individually cultured fusion products. Regeneration of plants occurs via embryogenesis and occasionally by polyembryony and organogenesis. Flowering plants can be obtained within 100 days of gamete fusion. Regenerated plants were studied by karyological and morphological analyses, and the segregation of kernel color was determined. The hybrid nature of the plants was confirmed.