Andreeva, Irena
[Verfasser:in]
;
Rodnina, Marina
[Akademische:r Betreuer:in];
Neumann, Heinz
[Sonstige Person, Familie und Körperschaft];
Stark, Holger
[Sonstige Person, Familie und Körperschaft]
Kinetic models of sequential initiation events upon polysome formation
Beschreibung:
Polysomes are active protein-synthesizing units consisting of multiple ribosomes assembled along one mRNA. The frequency of ribosome loading on the mRNA defines the number of ribosomes per polysome, which is an important regulator of the proteomic output from the transcriptome. The mechanisms that regulate the frequency of ribosome loading on the mRNA, and thus protein abundance are largely unknown. Here we have studied ribosome loading in real-time in vitro employing fluorescence resonance energy transfer (FRET), rapid kinetics, and global fitting approaches. We monitored how the first rib...