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Prigge, Elena-Sophie [VerfasserIn]; Tóth, Csaba [VerfasserIn]; Dyckhoff, Gerhard [VerfasserIn]; Freier, Kolja [VerfasserIn]; Plinkert, Peter K. [VerfasserIn]; Hoffmann, Jürgen [VerfasserIn]; Vinokurova, Svetlana [VerfasserIn]; Knebel Doeberitz, Magnus von [VerfasserIn]; Reuschenbach, Miriam [VerfasserIn]

p16INK4a/Ki-67 co-expression specifically identifies transformed cells in the head and neck region

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  • Medientyp: E-Artikel
  • Titel: p16INK4a/Ki-67 co-expression specifically identifies transformed cells in the head and neck region
  • Beteiligte: Prigge, Elena-Sophie [VerfasserIn]; Tóth, Csaba [VerfasserIn]; Dyckhoff, Gerhard [VerfasserIn]; Freier, Kolja [VerfasserIn]; Plinkert, Peter K. [VerfasserIn]; Hoffmann, Jürgen [VerfasserIn]; Vinokurova, Svetlana [VerfasserIn]; Knebel Doeberitz, Magnus von [VerfasserIn]; Reuschenbach, Miriam [VerfasserIn]
  • Erschienen: 2015
  • Erschienen in: International journal of cancer ; 136(2015), 7, Seite 1589-1599
  • Sprache: Englisch
  • DOI: 10.1002/ijc.29130
  • ISSN: 1097-0215
  • Identifikator:
  • Schlagwörter: E6*I RNA ; head and neck squamous cell carcinoma ; HPV ; Ki-67 ; p16
  • Entstehung:
  • Anmerkungen: Im Titel ist „INK4a“ hochgestellt
    Published online: 7 Aug 2014
  • Beschreibung: p16INK4a immunohistochemical overexpression is an overall reliable surrogate marker of human papillomavirus (HPV)-associated head and neck squamous cell carcinomas (HNSCC). However, cases of ambiguous p16INK4a overexpression are regularly detected in the head and neck: p16INK4a expression can be observed in non-malignant tissue, such as tonsillar crypt epithelium and a proportion of branchial cleft cysts. Additionally, diverse patterns of p16INK4 expression can complicate interpretation of “p16INK4a-positivity”. These aspects impede the unrestricted application of p16INK4a as a diagnostic marker in the head and neck. We hypothesized that combined detection of p16INK4a and the proliferation marker Ki-67 could support clarification of ambiguous p16INK4a expression in the head and neck by specifically indicating p16INK4a-expressing cells with proliferative activity. p16INK4a/Ki-67 co-expression in a combined staining procedure was correlated to distinct p16INK4a expression patterns and HPV status (HPV DNA followed by E6*I oncogene mRNA detection) in 147 HNSCC and 50 non-malignant head and neck samples. p16INK4a/Ki-67 co-expression only occurred in transformed cells of the head and neck. Co-expression was never detected in non-transformed cells. Combined p16INK4a/Ki-67 expression was stringently associated with a diffuse p16INK4a expression pattern. All HPV oncogene-expressing HNSCC showed p16INK4a/Ki-67 co-expression. We demonstrate that p16INK4a/Ki-67 co-expression occurs exclusively in transformed cells of the head and neck. Our findings indicate a substantial impact of combined p16INK4a/Ki-67 expression in the assessment of ambiguous p16INK4a expression in the head and neck by specifically identifying p16INK4a-expressing cells with proliferative activity. This property will be of considerable significance for head and neck histo- and cytopathology.
  • Zugangsstatus: Freier Zugang