Beschreibung:
Background: The aim of the presented thesis was to analyze the activation of the monomeric G protein RhoA in adult cardiomyocytes and further to unravel the role of the guanine nucleotide exchange factor p63RhoGEF in this context. Previous data demonstrated that RhoA is activated in neonatal cardiomyocytes by GPCR ligands like endothelin-1 (ET-1), phenylephrine (PE) and angiotensin II (AngII). So far, a contribution of the Gq/11 protein-regulated p63RhoGEF was demonstrated in the ET-1-mediated RhoA activation in neonatal cardiomyocytes. Moreover, RhoA was described to be permanently activat...