Dias de Sousa, Joana Manuela
[VerfasserIn]
;
Lalk, Michael
[AkademischeR BetreuerIn];
Eisenreich, Wolfgang
[AkademischeR BetreuerIn]Universität Greifswald
Insights into the metabolic responses of Bacillus subtilis to the absence of the key signal metabolites pyruvate and UDP-glucose
Hochschulschrift:
Dissertation, Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald, 2019
Anmerkungen:
Literaturverzeichnis: Seite 111-128
Beschreibung:
Biochemie
Using validated analytical tools and optimized sampling procedures, it was possible to detect a vast number of metabolites from the extracellular space but also from the cytosol of B. subtilis. The results indicate that the complement of the analytical methods was suitable in the monitoring of the metabolome since it allowed a great coverage of physicochemical diverse metabolites. However, a wide number of unknown metabolites/features were also detected. Although broad databases exist that can help in the annotation of metabolites, further investigation is needed in their identification. In unpredictable changing conditions, bacterial cells possess appropriate adaptation strategies for a successful bacterial growth. These rely on sensing mechanisms that globally adjust gene expression via transcription and feedback regulations. The metabolic sensing mechanisms have emerged as key roles in the nutritional information and regulation of cell cycle processes. In this work, a new quality of information regarding the metabolism and adaptation to the absence of key signal mechanisms in B. subtilis was provided. Investigations of cells lacking Pyk uncovered alterations in the import of glucose and pyruvate from the nutritional media. These results gives insights to the pyruvate homeostasis mechanism but also brought new questions concerning the regulation of the CCR. Pyruvate wasn't susceptible to the glucose dependent CCR in Δpyk. The earlier in ux of pyruvate in these cells is in ...