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Medientyp:
E-Book;
Hochschulschrift
Titel:
Ex-vivo-Untersuchung zum Einfluss der Plasmabehandlung von menschlicher Haut mittels Atmosphärendruck-Plasmajet kINPen® MED auf molekularer Ebene
Hochschulschrift:
Dissertation, Universitätsmedizin der Universität Greifswald, 2019
Anmerkungen:
Literaturverzeichnis: Seite 56-62
Beschreibung:
Plasma, Wundheilung
The application of low-temperature atmospheric pressure plasmas in the field of wound care is steadily gaining in importance and so the interest in the triggered biological processes in the organism is increasing. There are numerous studies on the influence of plasma on different cells in culture. In the present work we investigated the effect of plasma on an intact cell structure, the human skin on the molecular level. The atmospheric pressure plasma device KINPen® MED was used to treat skin samples ex vivo from a total of 9 patients. Using fluorescence microscopy the skin biopsies where evaluated for differentiation, proliferation, apoptosis and DNA double strang breaks 24 hours assessed after plasma exposure. Furthermore, the secretion of cytokines by ELISA were explored. The duration of the punctual plasma treatments was 1 minute, 3 minutes and 5 Minutes. The biggest limitations in the study design were the small sample size and the inhomogenecity of the experimental groups. There were no significant differences between treated and untreated skin sample. Despite that were observed important tendencies. The application of the plasma seems not to change the differentiation pattern of the epidermis. The longer treatment up to 5 minutes increased the number of basal apoptotic cells, while there were no changes in the DNA double-strand breakage and secretion of IL6 or IL8. The number of proliferative basal cells increased to a plasma exposure of 3 minutes. Short-term plasma ...