Hochschulschrift:
Dissertation, Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald, 2019
Anmerkungen:
Literaturverzeichnis: Seite 135-152
Zusammenfassung in englischer Sprache
Beschreibung:
Leader Protease, Maul- und Klauenseuche Virus
Foot-and-mouth disease (FMD) is an animal disease that causes dramatic economic losses to susceptible animals during FMD outbreaks. The causative agent of the disease is the foot-and-mouth disease virus (FMDV), which belongs to the family of Picornaviruses and the genus Aphthoviruses. Outbreaks of FMD are rare worldwide, but research on the virus should be continued in order to better understand the molecular and cellular processes involved. This will help to develop safe and efficient vaccines and take appropriate countermeasures in the case of an outbreak. The genome of the virus (consisting of approximately 8500 base pairs) codes for the leader protease, an important virulence factor that interferes with the expression of host proteins by cleavage of the eukaryotic initiation factor 4G (eIF4G) and furthermore influences the immune response of the infected host. In this thesis, different experimental systems for the investigation and differentiation of leader protease functions were analyzed. Using different experimental strategies (infection-, replicon-, & single expression-system) translation inhibition via eIF4G cleavage and its influence on other functions of the leader protease was investigated. The FMDVinduced cleavage of eIF4G could be verified for the infection- as well as for the repliconsystem. In the single expression-system, however, cleavage could only be seen after cell lysis. This experimental problem, which has not been described for FMDV before, questions ...