• Medientyp: E-Book; Hochschulschrift
  • Titel: Präklinische Untersuchungen zur Kombinationsbehandlung muriner Pankreaskarzinomzellen mit gewebeverträglichem Niedertemperaturplasma und Gemcitabin
  • Beteiligte: Behr-Negendanck, Magdalena von [Verfasser:in]; Partecke, Lars Ivo [Akademische:r Betreuer:in]; Vollmar, Brigitte [Akademische:r Betreuer:in]
  • Körperschaft: Universität Greifswald
  • Erschienen: Greifswald, 2020
  • Umfang: 1 Online-Ressource (PDF-Datei: 109 Seiten, 11539 Kilobyte); Illustrationen (teilweise farbig), Diagramme (teilweise farbig)
  • Sprache: Deutsch; Englisch
  • Identifikator:
  • RVK-Notation: XH 7504 : Dissertation, Habilitationsarbeit
  • Schlagwörter: Bauchspeicheldrüsenkrebs > Kaltes Plasma > Gemcitabin > Tumorzelle > Zellkultur > Tumorhemmung > Chemotherapie > In vitro
  • Entstehung:
  • Hochschulschrift: Dissertation, Universitätsmedizin der Universität Greifswald, 2020
  • Anmerkungen: Literaturverzeichnis: Seite 77-98
    Mit englischer Zusammenfassung
  • Beschreibung: Bauchspeicheldrüsenkrebs, Chemotherapie, Gemcitabin, Kaltes Plasma, Tumorzelle, anti-tumor treatment, cold plasma, gemcitabine, pancreatic cancer cells, plasma-jet, radicals, tumor cell selectivity

    Several groups have previously demonstrated anti-tumour effects with physical non-thermal, tissue tolerable plasma (TTP) treatment. Plasma is an ionized gas that develops its dose-depending, anti-proliferative effects by generating reactive oxygen and nitrogen species. However, it was shown that TTP exhibits cancer cell-selective killing property. Pancreatic cancer remains despite modern therapeutic approaches one of the most lethal cancers and is still a key challenge in the clinical practice. The most potent chemotherapeutic, Gemcitabine, prolongs the mean survival time only about two weeks. The combination of TTP with Gemcitabine could be an improving new supportive therapeutic approach in pancreatic cancer treatment. This work was conducted to evaluate the in vitro effects of TTP-treated cell culture medium in combination with Gemcitabine in murine 6606PDA pancreatic cancer cells and non-malignant C57BL/6 murine Fibroblasts. This combination therapy was significantly superior to mono-treatment in both cell lines investigated, as demonstrated by reduced metabolic activity and cell viability. 25 seconds TTP-treatment led to a reduction in cell viability of about 50 % in pancreatic cancer cells. Whereas Fibroblasts needed for the same decrease in cell viability a TTP-treatment of 70 seconds. The antitumor effect of TTP was significantly inhibited by N-acetyl cysteine in all the cells. These results suggested that ROS play a critical role in TTP treatment. To assess whether ...
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