Hochschulschrift:
Dissertation, Universitätsmedizin der Universität Greifswald, 2021
Anmerkungen:
Literaturverzeichnis: Seite 56-64
Beschreibung:
Schlaganfall, Tregs
After a cerebral artery is occlusion, there is a local reduction in blood flow and consequent death of tissue in the affected region and induction of local inflammation. This reaction in the patient can also be observed in experimental occlusion using tMCAO (transient middle cerebral artery occlusion). The effect of stroke does not exclusively affect brain tissue, but also leads to immunosuppression in the periphery: there is lymphocytopenia, increased apoptosis of splenocytes, and atrophy of lymphoid organs, such as the spleen. The immunologic consequences of stroke and specifically the functions of different leukocyte populations are the focus of current research. The role of Tregs, especially with advancing age, has not been clearly elucidated and was therefore further investigated in this study. In the present work, FoxP3+ Treg-depleted animals (Deregs) were compared with C57BL/6 (BL6) naïve and stroke-induced animals. The apoptotic density (number of apoptotic leukocytes of a population per mm2 area covered by this population) of selected lymphocyte populations was examined: CD3+/CD4+, CD3+/CD8+, Ly6G+ and CD11c+. The study is performed in the following design: 1) development of apoptosis over time 1 to 7 days or 7 to 14 days after stroke. This investigation revealed a trend toward higher apoptosis and variance in BL6 animals, as well as significances for individual populations and time points. 2) Differences between "young" and "old" animals were examined, with a ...