Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells

Medientyp: E-Book; Sonderdruck

Titel: Multiplexed histology analyses for the phenotypic and spatial characterization of human innate lymphoid cells

Beteiligte: Pascual-Reguant, Anna [VerfasserIn]; Köhler, Ralf [VerfasserIn]; Mothes, Ronja [VerfasserIn]; Bauherr, Sandy [VerfasserIn]; Hernandez, Daniela C. [VerfasserIn]; Uecker, Ralf [VerfasserIn]; Holzwarth, Karolin [VerfasserIn]; Kotsch, Katja [VerfasserIn]; Seidl, Maximilian [VerfasserIn]; Philipsen, Lars [VerfasserIn]; Müller, Werner [VerfasserIn]; Romagnani, Chiara [VerfasserIn]; Niesner, Raluca [VerfasserIn]; Hauser, Anja E. [VerfasserIn]

Erschienen: London: Nature Publishing Group, 2021

Umfang: 1 Online-Ressource (15 Seiten); Illustrationen, Diagramme; Supplementary information

Sprache: Englisch

DOI: 10.1038/s41467-021-21994-8

ISSN: 2041-1723

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Beschreibung: Abstract: Innate lymphoid cells (ILCs) emerge in the last few years as important regulators of immune responses and biological processes. Although ILCs are mainly known as tissue-resident cells, their precise localization and interactions with the microenvironment are still unclear. Here we combine a multiplexed immunofluorescence technique and a customized computational, open-source analysis pipeline to unambiguously identify CD127+ ILCs in situ and characterize these cells and their microenvironments. Moreover, we reveal the transcription factor IRF4 as a marker for tonsillar ILC3, and identify conserved stromal landmarks characteristic for ILC localization. We also show that CD127+ ILCs share tissue niches with plasma cells in the tonsil. Our works thus provide a platform for multiparametric histological analysis of ILCs to improve our understanding of ILC biology

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