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Blakowski, Tim
[VerfasserIn]
;
Woedtke, Thomas von
[AkademischeR BetreuerIn];
Emmert, Steffen
[AkademischeR BetreuerIn];
Schmidt, Anke
[AkademischeR BetreuerIn]
Universität Greifswald
Die Effekte einer Behandlung mit kaltem Atmosphärendruckplasma auf die Zell-Zell-Kommunikation und den Nrf2-Signalweg in dermalen Fibroblasten aus Maushaut
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- Medientyp: E-Book; Hochschulschrift
- Titel: Die Effekte einer Behandlung mit kaltem Atmosphärendruckplasma auf die Zell-Zell-Kommunikation und den Nrf2-Signalweg in dermalen Fibroblasten aus Maushaut
- Beteiligte: Blakowski, Tim [VerfasserIn]; Woedtke, Thomas von [AkademischeR BetreuerIn]; Emmert, Steffen [AkademischeR BetreuerIn]; Schmidt, Anke [AkademischeR BetreuerIn]
- Körperschaft: Universität Greifswald
-
Erschienen:
Greifswald, 2020
- Umfang: 1 Online-Ressource (PDF-Datei: 124 Seiten, 116188 Kilobyte); Illustrationen (teilweise farbig), Diagramme (teilweise farbig)
- Sprache: Deutsch
- Identifikator:
-
Schlagwörter:
Fibroblast
>
Zellkommunikation
>
Zellmigration
>
Oxidativer Stress
>
Connexin
>
Actin
>
Apoptosis
>
Zellskelett
>
Nuklearfaktor
>
Kaltes Plasma
>
Atmosphärendruckplasma
>
Haut
>
Maus
>
Tiermodell
- Entstehung:
- Hochschulschrift: Dissertation, Universitätsmedizin der Universität Greifswald, 2021
-
Anmerkungen:
Literaturverzeichnis: Seite 73-99
-
Beschreibung:
Fibroblast, Zellkommunikation, Zellmigration, Oxidativer Stress, Connexin, Apoptosis, Zellskelett, Haut, Kaltes Plasma, Maus, Aktin, Actin, CAP, Cx43, Keap1, Nrf2
The aim of this thesis was to explore the reaction of primary dermal fibroblasts, which were isolated from SKH1-mice, to a cold atmospheric-pressure plasma treatment using the argon-based plasma jet “kINPen MED” concerning their reaction to oxidative stress, their cell-cell communication via gap junctions and the organization of their actin cytoskeleton. The plasma treatment was administered in an indirect fashion by treating cell culture medium, which was afterwards used to incubate the cells. The results showed no significant induction of apoptosis by the indirect plasma treatment for treatment times between 20 s and 180 s, while the metabolic activity of cells was reduced significantly for up to 72 h in the cells that were plasma treated longer. This shows the correlation between plasma treatment time and cellular stress and at the same time their ability to compensate for this stress, protecting them from a possible increase in apoptosis after plasma treatment of up to 180 s. After plasma treatment an induction of the Nrf2 signaling pathway was shown, which functions as cellular safeguard against oxidative stress. The translocation of Nrf2 into the nucleus was demonstrated in primary fibroblasts as well as in skin tissue. An activation of the redox sensor Keap1 was shown as well, which binds Nrf2 physiologically and marks it for degradation in the proteasome. Another key point of this thesis was exploring the cell-cell communication via functional gap junctions. The ... - Zugangsstatus: Freier Zugang