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Kahan, Anat [VerfasserIn]; Greenbaum, Alon [VerfasserIn]; Jang, Min J. [VerfasserIn]; Robinson, J. Elliott [VerfasserIn]; Cho, Jounhong Ryan [VerfasserIn]; Chen, Xinhong [VerfasserIn]; Kassraian, Pegah [VerfasserIn]; Wagenaar, Daniel A. [VerfasserIn]; Gradinaru, Viviana [VerfasserIn]

'Light-Guided Sectioning' for Precise Localization and 3D Visualization of In Situ Optical Implants

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  • Medientyp: E-Book
  • Titel: 'Light-Guided Sectioning' for Precise Localization and 3D Visualization of In Situ Optical Implants
  • Beteiligte: Kahan, Anat [VerfasserIn]; Greenbaum, Alon [VerfasserIn]; Jang, Min J. [VerfasserIn]; Robinson, J. Elliott [VerfasserIn]; Cho, Jounhong Ryan [VerfasserIn]; Chen, Xinhong [VerfasserIn]; Kassraian, Pegah [VerfasserIn]; Wagenaar, Daniel A. [VerfasserIn]; Gradinaru, Viviana [VerfasserIn]
  • Erschienen: [S.l.]: SSRN, [2021]
  • Erschienen in: CELL-REPORTS-D-20-05829
  • Umfang: 1 Online-Ressource (40 p)
  • Sprache: Englisch
  • DOI: 10.2139/ssrn.3744424
  • Identifikator:
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: In the last two decades, there has been a significant increase in the use of implanted optical devices to control and monitor neuronal activity in vivo. However, post hoc examination of the implant location is commonly achieved via 2D histology, which often suffers from distortion and loss during tissue processing. To address this, we developed a 3D method that allows post hoc labeling and clearing of tissue that preserves the size of the tissue while leaving the optical implant unperturbed. By coupling the implant to a light-emitting diode, the implant itself can serve as a light guide for removal of redundant tissue (light-guided sectioning, LiGS). The residual tissue, including the tissue–implant interface, is then processed as a whole for further investigation. This enables the precise location of the optical implant to be determined as well as the identification of tissue changes at the implant site. LiGS can also be used for accurate cell registration of ex vivo histology with single-cell two-photon calcium images obtained through gradient-index (GRIN) lenses. We anticipate that LiGS will provide valuable additional information in any experiment that uses optical implants and will increase reproducibility through well-defined fiber-to-target localization
  • Zugangsstatus: Freier Zugang