A Smart Small Molecule as Specific Fluorescent Probe for Ultrasensitive Recognition of Mitochondrial G-Quadruplexes

Medientyp: E-Book

Titel: A Smart Small Molecule as Specific Fluorescent Probe for Ultrasensitive Recognition of Mitochondrial G-Quadruplexes

Beteiligte: Wang, Yakun [Verfasser:in]; Chen, Niping [Verfasser:in]; Pan, Zhenxing [Verfasser:in]; Ye, Zhaoyi [Verfasser:in]; Yuan, Jiongpeng [Verfasser:in]; Zeng, Yaoxun [Verfasser:in]; Long, Wei [Verfasser:in]; Bian, Wangqing [Verfasser:in]; Li, Xiaojing [Verfasser:in]; Lu, Yujing [Verfasser:in]; Zhang, Kun [Verfasser:in]; He, Yan [Verfasser:in]; Liu, Xujie [Verfasser:in]

Erschienen: [S.l.]: SSRN, [2022]

Umfang: 1 Online-Ressource (22 p)

Sprache: Englisch

DOI: 10.2139/ssrn.4013486

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Beschreibung: G-quadruplex (G4) has become an essential target for the therapy of human genetic diseases (cancer included) with important biological functions for regulation and monitoring. While little is known about G-quadruplexes in mitochondria due to the limited development of fluorescent ligands for mitochondrial DNA (mtDNA) G4 detection, urging the design of the fluorogenic detection ligands. Herein, a robust aggregation-induced emission luminescent ligand, SPN , was designed and synthesized for the recognition of mtDNA G4s. Using SPN as the mtDNA G4s sensor, we surprisingly found that SPN could achieve ultrasensitive recognition, sensing, and stabilization of the G4s with the detection limit as low as 0.89 nM. SPN can be highly colocalized with mitochondria, allowing monitoring of mtDNA G4s effectively in vitro. Remarkably, the fluorescent intensity in cancer cells is approximately 3-fold that in normal cells, suggesting the potential applications of SPN in cancer diagnosis. This study provides a promising example of designing a fluorescent probe targeting and sensing mtDNA G4s and offers a feasible chemical tool for investigating mtDNA G4s

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