Beschreibung:
Protein availability is crucial for maintaining cellular processes. In synapses, the identities and functions of proteins involved in neurotransmission are well known. Their spatial distribution, copy numbers, and lifetimes have also been thoroughly investigated. However, little information is available on their mobility and its regulation. In this thesis, I have addressed this issue by analyzing the mobility of 45 diverse proteins in the synapses of primary hippocampal neurons. Relying on the fluorescence recovery after photobleaching (FRAP) technique, I have measured the mobility of prote...