Wenske, Sebastian
[VerfasserIn]
;
Lalk, Michael
[AkademischeR BetreuerIn];
Benedikt, Jan
[AkademischeR BetreuerIn];
Wende, Kristian
[AkademischeR BetreuerIn]Universität Greifswald
Mass spectrometric analysis of non-enzymatic post-translational modifications introduced by cold plasma-derived reactive species in peptides and proteins
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Titel:
Mass spectrometric analysis of non-enzymatic post-translational modifications introduced by cold plasma-derived reactive species in peptides and proteins
The relevance of cold atmospheric plasmas (CAPs) in biomedicine has recently grown. The potential of CAPs has been discussed in multiple scientific works, highlighting its effectiveness in promoting wound healing, limiting cancer progression, and for sterilization of surfaces. Main bioactive molecules, such as reactive oxygen and nitrogen species (RONS), are proposed as key candidates in these processes. Indeed, the generation of cold plasma induces noble gas ionization which, reacting with atmospheric air molecules, generates species such as singlet oxygen, atomic oxygen radicals, nitric oxide radicals. Although molecular simulations have been conducted, the mechanism of action on biological molecules, as well as the possibility to tune plasmas to produce specific species cocktails (e.g., with different degree of oxidation power) has been not fully unleashed. In this dissertation, presented in form of 5 published scientific articles, focus has been placed on the interaction of plasmas with peptides and proteins, which are main biological effectors in cellular compartments. Precisely, through the development of liquid chromatography coupled mass spectrometry (LC-MS) methods, the effects of plasmas on peptides and proteins in form of oxidative post-translational modifications (oxPTMs) has been investigated. The characterization of these oxPTMs has been performed by treating peptide or protein aqueous solutions and on porcine skin tissues. It has been found that, introducing ...