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Borkute, Rachana
[Verfasser:in]
;
Dorhoi, Anca
[Akademische:r Betreuer:in];
Jantsch, Jonathan Antti Michael
[Akademische:r Betreuer:in]
Universität Greifswald
Responses of bovine and human neutrophils to members of the Mycobacterium tuberculosis complex
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- Medientyp: E-Book; Hochschulschrift
- Titel: Responses of bovine and human neutrophils to members of the Mycobacterium tuberculosis complex
- Beteiligte: Borkute, Rachana [Verfasser:in]; Dorhoi, Anca [Akademische:r Betreuer:in]; Jantsch, Jonathan Antti Michael [Akademische:r Betreuer:in]
- Körperschaft: Universität Greifswald
-
Erschienen:
Greifswald, 20.07.2022
- Umfang: 1 Online-Ressource (PDF-Datei: 160 Seiten, 5383 Kilobyte); Illustrationen (farbig), Diagramme (teilweise farbig)
- Sprache: Englisch
- Identifikator:
-
RVK-Notation:
WF 6750 : Mycobacteriaceae
YE 1604 : Dissertation, Habilitationsarbeit
XX 1304 : Dissertation, Habilitationsarbeit
-
Schlagwörter:
Rindertuberkulose
>
Zoonose
>
Tuberkulose
>
Tuberkelbakterium
>
Mycobacterium bovis
>
Mycobacterium
>
Neutrophil Extracellular Trap
>
Neutrophiler Granulozyt
>
Immunologie
>
Hochschulschrift
>
Dissertation
- Entstehung:
-
Hochschulschrift:
Dissertation, Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald, 2023
-
Anmerkungen:
Literaturverzeichnis: Seite 120-153
-
Beschreibung:
Bovine TB, neutrophils, immunology, zoonotic TB
PMN are one of the most important cells of the innate immune system and are responsible for fast clearance of invading pathogens in most circumstances. The role of human PMN during mycobacterial infection have been widely studied. Nevertheless, there are contradicting results regarding their role in protection or pathology during TB. Similar studies focusing on bovine PMN and their role in M. bovis infection remain understudied. Also, not much is known about attenuation of M. tb in cattle and responses of PMN to this MTBC member. The major aims of this study were to i) gain insights into bovine PMN biology and the cellular processes triggered by challenge with virulent mycobacteria and to ii) find out whether interspecies differences result in different outcomes upon in vitro challenge. In the first part of the work, a new isolation method for bovine PMN from whole blood was developed. Human and bovine PMN have different buoyant properties and hence need to be isolated using different procedures. The magnetic isolation method developed within this thesis is robust and results in very good yields of highly pure, viable bovine PMN populations. This is extremely advantageous and indispensable for downstream functional assays that are required to be performed on a single day. The second goal of this study was to compare and contrast the functional differences between bovine and human PMN upon BCG infection. The findings reveal for the first time that human PMN phagocytose more ... - Zugangsstatus: Freier Zugang