Hochschulschrift:
Dissertation, Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald, 2023
Anmerkungen:
Literaturverzeichnis: Seite 180-213
Beschreibung:
Massenspektrometrie, Zoonose, Viren, Zellkultur, Proteine, Cedar virus, Ebola virus, Interactomes, Junin virus, Lloviu virus, Mass spectrometry, Nipah virus, Reston virus, Tacaribe virus, Viurs-host protein interaction
Emerging zoonotic viruses are a constant threat to human and animal health. Therefore, knowledge about the host factors influencing viral pathogenicity is highly welcome as a basis for developing treatment or vaccine strategies. In order to identify host factors that potentially determine the pathogenicity of three highly pathogenic (’high consequence’) zoonotic viruses, the interactomes of selected viral proteins were analysed in parallel with the interactomes of the homologous proteins from closely related viruses which lack high pathogenicity. For this purpose, affinity purification mass spectrometry (AP-MS) was performed with the virus proteins as baits and lists of candidate proteins were generated that may determine the pathotype and warrant follow-up studies to characterise their function concerning the viral life cycles. In detail, the interactomes of virus pairs from the arenaviruses, filoviruses and henipaviruses were studied. The following protein homologues were selected: for filoviruses, the transcription factor VP30, the co-transcription factor VP35 and matrix protein VP40 of the non-pathogenic Reston virus (RESTV, species Reston ebolavirus), the pathogenic Ebola virus (EBOV, species Zaire ebolavirus), and, in addition, the Lloviu virus (LLOV, species Lloviu cuevavirus); in case of the arenaviruses the nucleoprotein (NP), matrix protein (Z) and glycoprotein (GP) of the pathogenic Junín virus (JUNV, species Argentine mammarenavirus) and the non-pathogenic ...