Identifizierung und Charakterisierung einer Lipase (ALIP2) und zweier Carboxylesterasen (BEST1 und BEST2) aus der Hefe Blastobotrys raffinosifermentans LS3
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Identifizierung und Charakterisierung einer Lipase (ALIP2) und zweier Carboxylesterasen (BEST1 und BEST2) aus der Hefe Blastobotrys raffinosifermentans LS3
Polymers are widely used in our everyday lives. In the form of functional polymers, they are used, among other things, as active ingredients or effect substances. They consist of a carrier to which a functional group is bonded via a spacer. The spacer groups influence the chemical, physical and biological properties of the polymers or make them possible in the first place. As a result, they are key building blocks in the pharmaceutical industry and medicinal chemistry. Monoesters of symmetrical dicarboxylic acids or symmetrical diols are also used for the introduction of spacer groups. They can be chemically synthesized by the hydrolysis of diesters or diol diesters. Since this reaction is not selective, by-products such as diacids or diols are formed, which reduce the yields and require expensive work-up. Selective enzymatic processes represent a real alternative, because it is not necessary to separate the product from the by-product. To date, only a few enzymes are known and available that can be used for the synthesis of monoesters. The aim of this work is to discover new lipases and carboxylesterases as biocatalysts for the synthesis of monoesters, which should also be generated in sufficient availability. The yeast Blastobotrys raffinosifermentans served as gene donor and expression system for this purpose. The non-conventional, non-pathogenic and thermotolerant yeast B. raffinosifermentans has a broad carbon and nitrogen source spectrum, which makes it interesting ...