Triplet-encoded prebiotic RNA aminoacylation

Medientyp: E-Artikel
Titel: Triplet-encoded prebiotic RNA aminoacylation
Beteiligte: Su, Meng [Verfasser:in]; Schmitt, Christian [Verfasser:in]; Liu, Ziwei [Verfasser:in]; Roberts, Samuel J. [Verfasser:in]; Liu, Kim C. [Verfasser:in]; Röder, Konstantin [Verfasser:in]; Jäschke, Andres [Verfasser:in]; Wales, David J. [Verfasser:in]; Sutherland, John D. [Verfasser:in]
Erschienen: July 26, 2023
Erschienen in: American Chemical Society: Journal of the American Chemical Society ; 145(2023), 29 vom: Juli, Seite 15971-15980
Sprache: Englisch
DOI: 10.1021/jacs.3c03931
Identifikator:

Entstehung:
Anmerkungen: Online verfügbar: 12. Juli 2023
Beschreibung: The encoding step of translation involves attachment of amino acids to cognate tRNAs by aminoacyl-tRNA synthetases, themselves the product of coded peptide synthesis. So, the question arises─before these enzymes evolved, how were primordial tRNAs selectively aminoacylated? Here, we demonstrate enzyme-free, sequence-dependent, chemoselective aminoacylation of RNA. We investigated two potentially prebiotic routes to aminoacyl-tRNA acceptor stem-overhang mimics and analyzed those oligonucleotides undergoing the most efficient aminoacylation. Overhang sequences do not significantly influence the chemoselectivity of aminoacylation by either route. For aminoacyl-transfer from a mixed anhydride donor strand, the chemoselectivity and stereoselectivity of aminoacylation depend on the terminal three base pairs of the stem. The results support early suggestions of a second genetic code in the acceptor stem.
Zugangsstatus: Freier Zugang

Nur in Feld suchen: